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KMID : 0861020130280010043
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Korea Journal of Herbology
2013 Volume.28 No. 1 p.43 ~ p.50
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The Study of Anti-inflammatory Effect of Hwanggeumjakyak-tang Extract in RAW 264.7 Macrophage
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Kim Ma-Ryong
Kang Ok-Hwa
Kim Sung-Bae
Kang Hee-Jung
Kim Ji-Eun
Hwang Hyeong-Chil
Kim In-Won
Kwon Dong-Yeul
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Abstract
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Objectives : Hwanggeumjakyak-tang (huangqin shaoyao tang, HJT) has been used to treat acute enteritis in traditional oriental medicine. However, there has been a lack of studies regarding the effects of HJT on the inflammatory activities and effector inflammatory disease mechanism about macrophage before is not known. So we examined the effect of HJT water extract on pro-inflammatory mediators in lipopolysaccharide (LPS) - stimulated mouse macrophage, RAW 264.7 cells.
Methods : Cells were treated with 2 ug/mL of LPS 1 h prior to the addition of HJT. Cell viability was measured by MTS assay. The production of nitric oxide (NO) was determined by reacting cultured medium with Griess reagent. The xpression of cyclooxygenase-2 (COX-2), inducible NO synthase (iNOS) and mitogen-activated protein kinases (MAPKs) was investigated by Western blot, RT-PCR. The content of level of cytokines (prostaglandin (PG) E₂, interleukin (IL)-6, IL-12, Tumor necrosis factor-alpha (TNF-α) and monocyte chemoattractant protein-1 (MCP-1)) in media from LPS-timulated Raw 264.7 cells was analyed by ELISA kit.
Results : HJT inhibited the production of NO, PGE₂, IL-6 as well as the expressions of iNOS, COX-2 but did not inhibit the production of IL-12, TNF-α, MCP-1 in the murine macrophage, RAW 264.7 cells. HJT also had suppression effects of LPS- induced MAPKs activation
Conclusion : These results suggest that HJT has an anti-inflammatory therapeutic potential, which may result from inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes.
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KEYWORD
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Hwanggeumjakyak-tang, Anti-inflammation, ELISA, Western blot, RT-PCR
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